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. 2018 Jul 13;14(10):1221–1231. doi: 10.7150/ijbs.25488

Figure 2.

Figure 2

Augmented cell death and suppressed cell proliferation in SCLC. A, B: H69 and H82 cells were treated with AZD1775 or MLN0128 alone or combination for 48 hours at indicated concentrations, followed by Annexin-V and PI staining. Cells were analyzed by flow cytometry and the percentage of Annexin V+, PI- (early apoptotic) and Annexin-V+, PI+ (late apoptotic) cells were calculated. Data represent mean ± SEM of total apoptotic cell population in quadruple experiments. ****p < 0.0001 compared treatments among the same group; ## p < 0.01, #### p < 0.0001 compared MLN0128-treated cells to combination-treated cells. C, D: H69 or H82 cells were treated with combination of AZD1775 (1000 nM for H69, 250 nM for H82) and MLN0128 (50nM) for 48 hours, followed by BrdU and 7-AAD staining and flow cytometry analysis. Stacked bars show percentage of SCLC cell population in sub-G1, G1, S, and G2 cell cycle phases. Data are presented as the mean ± SEM of quadruple experiments.