Figure 4.

ER stress-induced SCLC cell apoptosis is CHOP dependent. A. H69 and H82 cells were transduced with recombinant lenti-viruses expressing a control shRNA targeting fire fly luciferase (LUC-sh) or shRNA-CHOP #1 or #2 and incubated for 48 hours. Transfected cells were treated with AZD1775 (1000nM for H69, 250nM for H82) and MLN0128 (50nM) for 48 hours, expression of CHOP was determined by Western blot analysis; Tubulin was used as a loading control. B, C. Transfected H69 and H82 cells were treated with MLN0128 (50nM) and AZD1775 (1000nM for H69, 250nM for H82) followed by Annexin-V and PI staining and flow cytometry analysis. Data are presented as the mean ± SEM of quadruple experiments. ****p < 0.0001 compared to cells transfected with control shRNA.