FIGURE 1.

Ethanol treatment induces caspase-1 activation. A. HEECs were treated with ethanol at the concentrations of 0, 0.5%, 1%, 2%, 4%, 8% and 16%, and the cellular survival rate was detected by the MTT assay. B. HEEC cells were separately treated with 0.5% ethanol and 1% ethanol, and caspase-1 activation, as well as its mRNA and protein expression levels, were detected by immunofluorescence (B), real-time PCR (C) and western blotting (D). The data are representative of five independent experiments and represent the means ± SD. **, p<0.01; ***, p<0.001. ^###, p<0.001. * indicates the comparison between adjacent groups, ^# indicates the comparison between the groups independent of each other.