Table 1. Primers used in the current study (A).

A
No.	Gene	F/R	Sequence	Genomic position	Exon-intron junction	Intron spanning	Amplicon size (bp)	Reference
1	RPL27	F	ACAATCACCTAATGCCCACA	chr17:43000081-43002884	√	√	146	This study
		R	GCCTGTCTTGTATCTCTCTTCAA
2	RPS29	F	GCACTGCTGAGAGCAAGATG	chr14:49577820-49586363	√	√	213	De Jonge et al. (2007)
		R	ATAGGCAGTGCCAAGGAAGA
3	OAZ1	F	GGATCCTCAATAGCCACTGC	chr19:2269621-2271410	–	√	150	De Jonge et al. (2007)
		R	TACAGCAGTGGAGGGAGACC
4	RPL30	F	ACAGCATGCGGAAAATACTAC	chr8:98041745-98042696	–	√	158	De Jonge et al. (2007)
		R	AAAGGAAAATTTTGCAGGTTT
5	LDHA	F	TTGTTGGGGTTGGTGCTGTT	chr11:18396916-18399514	–	√	137	This study
		R	AAGGCTGCCATGTTGGAGAT
6	ACTB	F	CCTTGCACATGCCGGAG	chr7:5529603-5530574	–	√	112	https://primerdepot.nci.nih.gov
		R	GCACAGAGCCTCGCCTT
7	RPS13	F	CGTCCCCACTTGGTTGAAGTT	chr11:17075584-17077438	√	√	129	This study
		R	CGTACTTGTGCAACACCATGT
8	GAPDH	F	GCATCCTGGGCTACACTGA	chr12:6537873-6538138	–	√	162	Campos et al. (2009)
		R	CCACCACCCTGTTGCTGTA
9	TUBA1B	F	GTCGCCTTCGCCTCCTAATC	chr12:49129610-49131330	–	√	146	This study
		R	TCACTTGGCATCTGGCCATC
10	PSMC5	F	TTGACGGACCAGAGCAGATG	chr17:63827498-63829528	√	√	124	This study
		R	CTCCGGAGGTTTTGGCTCTT
11	MTCH1	F	CTTGGCGTAGGTGAAGAAGC	chr6:36978607-36985885	√	√	123	https://primerdepot.nci.nih.gov
		R	CATCCCCTGCTCTACGTGAA
12	MKRN1	F	GCAGCAAGGGATGACTTTGT	chr7:140459862-140471905	–	√	98	https://primerdepot.nci.nih.gov
		R	TGTATTTATGGAGACCGCTGC
13	ERGIC3	F	TCTCATGCTGCTACTGTTCCT	chr20:35542342-35542833	√	√	152	https://pga.mgh.harvard.edu/primerbank/
		R	CAATACTCAGATAGGCACAAGGC
14	ADRM1	F	CAATGCTCCTCATCCTGGTC	chr20:62304519-62306228	–	√	91	https://primerdepot.nci.nih.gov
		R	GGAGGGTCTACGTGCTGAAG
15	RPL37A	F	TTCTGATGGCGGACTTTACC	chr2:216499955-216501378	–	√	115	https://primerdepot.nci.nih.gov
		R	ATGAAGAGACGAGCTGTGGG
16	RPL5	F	TCGTATAGCAGCATGAGCTTTC	chr1:92837537-92840589	–	√	136	https://primerdepot.nci.nih.gov
		R	TGTTGCAGATTACATGCGCT
17	TSPAN31	F	GGCTATTAACCGAAGCAAACAGA	chr12:57746235-57746683	–	√	117	https://pga.mgh.harvard.edu/primerbank/
		R	GTGAGGTTGAATAAGCCACAACA
18	DARS	F	AGCCGCAAGAGTCAGGAGA	chr2:135979349-135985450	–	√	124	https://pga.mgh.harvard.edu/primerbank/
		R	CCCGAACCAAAACTCGATCTG

Note:

Results from different tools on the internal control genes and their ranks as obtained using the validation samples (B).