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. Author manuscript; available in PMC: 2019 May 18.
Published in final edited form as: Chembiochem. 2018 Apr 26;19(10):1031–1035. doi: 10.1002/cbic.201800121

Figure 2.

Figure 2.

A. The BCL9 24-mer peptide. Underlined residues were chosen for substitution to propargyl serine to form the i, i+4 diyne staple. The peptide was synthesized on Rink Amide ChemMatrix resin. B. Reaction conditions for on-resin Glaser stapling. The displayed helix is the wild-type BCL9 24-mer, PDB entry 2GL7. C. From top to bottom, HPLC traces of crude peptide cleaved in 10% DMS, 2.5% TIPS, 2.5% H2O cocktail (2 hours) after Glaser stapling reaction at t=0, t=24 (76% conversion by peak area), and t=72 hrs (>95% conversion by peak area).