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. Author manuscript; available in PMC: 2019 May 18.
Published in final edited form as: Chembiochem. 2018 Apr 26;19(10):1031–1035. doi: 10.1002/cbic.201800121

Figure 3.

Figure 3.

CD spectra of diyne-stapled BCL9 24-mers versus unstapled linear BCL9 24-mers. All peptides were dissolved in PBS, pH 7.4, to a final concentration of 40 μM. 3 baseline scans of buffer were taken and averaged at T=25°C, followed by 5 scans of each peptide. These spectra were averaged, baseline-subtracted from the averaged blank scans, and converted to mean residue ellipticity to plot against wavelength. The percent helicity of each peptide was calculated based on [θ]222/[θ]max. [θ]max was calculated according to the formula: [θ]max=−39000(1–3/n), where n is the number of amide bonds.