Fig 5. In-vivo tumor growth inhibited by combination in inflammatory subtype but not in stem-like.

(A) (B): NCI-H747 and (C) (D): SW480 cells, respectively, were implanted into athymic nude mice. Once xenografts reached ~100 mm³, mice were grouped into 4 different cohorts (7 mice/cohort) (control [untreated], neratinib-only (10mg/kg), MEK162-only (3 mg/kg), or MEK162 plus neratinib groups). MEK162 and neratinib were suspended in 0.5% carboxymethyl cellulose and orally administered q.d. for 28 days, respectively. Tumor volumes were measured three times weekly. Volumes represent the mean ± SD of tumor volumes from 7 mice/group. Respective body weights were measured once weekly.