FIGURE 3.

Identification of mispriming events in RNA-seq data sets. (A) Schematic comparing bonafide cDNA peaks with peaks from mispriming events. RNA molecules that are properly ligated and reverse transcribed from specific RT primer-3′ adapter interaction produce a pile-up of cDNA reads that have staggered ends (left). On the other hand, when RT-primer pairs with a sequence similar to the 3′ adapter present within an RNA molecule, cDNA peaks with flush ends next to the priming site are produced. (B) Pipeline to identify sites of mispriming.