Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep;24(9):1144–1157. doi: 10.1261/rna.067165.118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Munir et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

FIGURE 7. — Step-specific effects of mutations on the kinetics of 2′-PO₄ removal. Assays were performed as described in the legend to Figure 6. The reaction products were analyzed by urea-PAGE and quantified by scanning the gels. The distributions of the 2′-P-ADPR RNA intermediate and 2′-OH RNA product (expressed as percentage of total RNA) are plotted as a function of reaction time. Each datum in the graphs is the average of three independent time course experiments ±SEM. The apparent rate constants for step1 shown in the table at bottom right were derived by plotting the sum of the 2′-P-ADPR RNA intermediate and 2′-OH RNA product as a function of time and fitting the data by nonlinear regression to a one-phase association in Prism.