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. Author manuscript; available in PMC: 2018 Aug 17.
Published in final edited form as: Cond Med. 2018 Feb 15;1(2):57–63.

Figure 2.

Figure 2

Primary astrocyte cultures from WT and Par-1 KO mice were pretreated with two doses of thrombin (0.5 and 0.1 U/ml) for 24 h and then collected for immunofluorescence staining and Western blot analysis. (A) Phosphorylated p44/42 MAPK immunoreactivity in astrocytes 24 h after control or thrombin (0.5, 1.0U/ml) treatment. (B) Western blot showing phosphorylated (P-) and total (T-) p44/42 MAPK protein levels in astrocytes after control or thrombin treatment for 24 h. Values are mean ± SD, *p<0.05 vs. the WT control group and KO TPC group. Scale bar = 50 μm.