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. Author manuscript; available in PMC: 2018 Aug 17.
Published in final edited form as: Cond Med. 2018 Feb 15;1(2):57–63.

Figure 3.

Figure 3

Primary astrocytes cultures from WT and Par-1 KO mice were pretreated with 0 (control), 0.5 or 1.0 U/ml thrombin for 24 h and then collected for immunofluorescence staining and Western blot analysis. (A) Immunoreactivity of phosphorylated p90RSK (T359/S363) in astrocytes 24 h after control or thrombin treatment. (B) Western blots showing phosphorylated (P-) and total (T-) p90RSK protein levels in astrocytes after control or thrombin treatment. Values are mean ± SD, *p<0.05 vs. the control and KO thrombin groups. Scale bar = 50 μm.