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Primary astrocytes cultures from WT and Par-1 KO mice were pretreated with 0 (control), 0.5 or 1.0 U/ml thrombin for 24 h and then collected for immunofluorescence staining and Western blot analysis. (A) HSP25 immunoreactivity in astrocytes after control or thrombin treatment. (B) Western blot showing HSP25 protein levels in astrocytes after control or thrombin treatment. Values are mean ± SD, #p<0.01 vs. Control group and KO thrombin treatment group. Scale bar = 50 μm.
