Video 5. Larval MDNs persist into adulthood and induce backward walking.

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DOI: 10.7554/eLife.38554.019

Walking behavior of adult flies carrying all the components showed in 8F (split3, right) or all the genetic components except the DBD half of Split3 (control, left). During the first 10 s, the animals are not under optogenetic light followed by 10 s under 0.28 mW/mm² of red light.