Fig. 4.

MPS concentration-response relationship using a worm TDP-43 proteinopathy model. Body bend frequencies of chronically-treated, age-synchronised TDP-43(A315T)-expressing worms were assessed to determine the concentration-response relationship of the compound at various ages of adulthood. (A) Body bends of control worms treated with the 0.4% DMSO vehicle at all ages. Although control values were not incorporated into concentration-response curves on the logarithmic scale as a logarithmic value cannot be obtained for 0 M of the compound, body bend frequencies for each day were compared against respective control values for statistical analysis. Locomotion defects were not ameliorated at any concentration on (B) days 1 and (C) 3 (p > .05). (D - F) At older ages, locomotion rate was significantly improved at concentrations of −4.6 M (0.025 mM) and −4.3 M (0.05 mM) (p < .05), although higher concentrations reduced it instead. Data shown was pooled from three biological replicates, and comparisons were made with the Kruskal-Wallis test with Dunn's multiple comparisons (n = 30 worms per day per concentration); *, p < .05.