Fig. 7.

Representative images of worms expressing GFP in GABAergic neurons. Age-synchronised wild type worms expressing GFP under the control of the unc-25 promoter are shown at adult ages of (A) day 3 and (B) day 7 to compare the appearance of D-type GABAergic motor neurons in the ventral nerve cord. The 19 cell bodies of this neuronal subset manifest as fluorescent punctae (indicated with arrows in A). (B) Ageing did not cause observable physical neuronal deterioration, which was evident from intact neuronal tracts and discernible cell bodies at adult age day 7. Images were taken at 200× magnification, scale bars = 100 μm. (C—F) Age-synchronised TDP-43(A315T)-expressing worms that also express GFP under the control of the unc-25 promoter were chronically treated with (A) 8 mM succinimide, (B) 8 mM ethosuximide, (C) 0.4% DMSO vehicle or (D) 0.05 mM MPS in 0.4% DMSO from the L1 larval stage. Breaks within the D-type GABAergic motor neuron tracts in the ventral nerve cord are indicated by red boxes, which are magnified in the inset red boxes. Images were taken at 200× magnification, scale bars = 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)