REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-CD45 (clone 2D1) (APC)	Thermo Fischer Scientific	Cat# 17-9459-42, RRID:AB_10718532
Rat monoclonal anti-CD4 (PE vio770)	Miltenyi Biotec	Cat# 130-102-784 RRID:AB_2659911
Rat monoclonal anti-CD8a (Vioblue)	Miltenyi Biotec	Cat# 130-102-431 RRID:AB_2659889
Rat monoclonal Anti-Gr1 (Vioblue)	Miltenyi Biotec	Cat# 130-102-233, RRID:AB_2659865
Rat monoclonal Anti-Cd11b (APC-vio770)	Miltenyi Biotec	Cat# 130-096-834, RRID:AB_2660135
Rat monoclonal anti-CD4 (clone GK1.5) (PE Cy7)	Thermo Fischer Scientific	Cat# 25-0041-81, RRID:AB_469575
Rat monoclonal anti-CD8a (clone 53-6.7) (APC Cy7)	Thermo Fisher Scientific	Cat# 47-0081-82, RRID:AB_1272185
Armenian hamster monoclonal anti-TCR-b (clone H57-597) (PE)	Thermo Fischer Scientific	Cat# 12-5961-82 RRID:AB_466066
Mouse monoclonal anti-phospho-Stat5 (pY694) (PerCPCy5.5)	BD Biosciences	Cat# 560118, RRID:AB_1645551
Rabbit monoclonal anti-Phospho-Stat5 (Tyr694) (C11C5)	Cell Signaling Technology	Cat# 9359S, RRID:AB_823649
Rabbit polyclonal anti-Stat5	Cell Signaling Technology	Cat# 9363, RRID:AB_2196923
Mouse monoclonal Anti-β–Actin (Clone AC-15)	Merck	Cat# A5441, RRID:AB_476744
Rabbit polyclonal anti-Stat5 (C17)	Santa Cruz Biotechnology	Cat# sc-835 RRID:AB_632446
Mouse monoclonal anti-Stat5 (A-9)	Santa Cruz Biotechnology	Cat# sc-74442 RRID:AB_1129711
Rabbit monoclonal anti-Stat5 (L-20)	Santa Cruz Biotechnology	Cat# sc-1081 RRID:AB_632448
Rabbit polyclonal anti-HOX11 (C18)	Santa Cruz Biotechnology	Cat# sc-880 RRID:AB_2203789
Rabbit polyclonal anti-MYC	Santa Cruz Biotechnology	Cat# sc-764 RRID:AB_631276
Rabbit anti-EP300	Santa Cruz Biotechnology	Cat# SC-585X RRID:AB_2616339
Rabbit polyclonal anti-BRD4	Bethyl	Cat# A301-985A50 RRID:AB_2631449
Mouse monoclonal anti-ETS1 (8A8)	Thermo Fischer Scientific	Cat# MA5-15609, RRID:AB_10978854
Rabbit polyclonal anti-RUNX1	Active Motif	Cat# 39000
Rabbit anti-H3K4me1	Active motif	Cat# 39297, RRID:AB_2615075
Rabbit monoclonal anti-H3K4me3 (clone C42D8)	Cell Signaling Technology	Cat# 9751, RRID:AB_2616028
Rabbit polyclonal anti-H3K27ac	Abcam	Cat# ab4729 RRID:AB_2118291
Rabbit polyclonal Anti-H3K27me3	Millipore	Cat# 07-449, RRID:AB_310624
Rabbit monoclonal anti- Cre Recombinase (D7L7L) XP®	Cell Signaling Technology	Cat# 15036
Goat polyclonal anti-CD3 epsilon	Santa Cruz Biotechnology	Cat# Sc-1127; RRID:AB_631128
Biological Samples
Patient-derived xenografts (PDX)	This paper
Chemicals, Peptides, and Recombinant Proteins
Imatinib (Gleevec)	Selleck Chemicals	Cat# S2475; CAS ID:152459-95-5
JQ1	Selleck Chemicals	Cat# S7110; CID ID: 1268524-71-5
ABT-199	MedChem Express	Cat# HY-15531; CAS ID: 1257044-40-8
iBET-762	Merck	Cat# SML1272; CAS ID: 1260907-17-2
CPI0610	Axon Med Chem	Cat# 2594; CAS ID: 1380087-89-7
DMSO	Millipore	Cat# 102950; CAS ID: 67-68-5
Polybrene Infection / Transfection Reagent	Merck	Cat# TR-1003-G
Recombinant murine IL7	Peprotech	Cat# 217-17
Recombinant murine IL6	Peprotech	Cat# 216-16
Recombinant murine IL3	Peprotech	Cat# 213-13
Recombinant murine SCF	Peprotech	Cat# 250-03
DLL4-Fc	Bornschein et al., 2018
Mouse monoclonal anti-Human IgG1 Fc	Abcam	Cat# ab1927; RRID:AB_956021
1M Tris-HCI, pH 8.0	Thermo Fischer Scientific	Cat# 15568025
Sodium dodecyl sulphate	VWR Chemicals	Cat# 444464T; Cas ID 151-21-3
Sodium deoxycholate	Merck	Cat# D6750; Cas ID: 302-95-4
Glycerol	Thermo Fischer Scientific	Cat# 15514-011
IGEPAL® CA-630	Merck	Cat# I8896; CAS ID: 9016-45-9
Triton™ X-100	Merck	Cat# T8787 CAS ID: 9002-93-1
Tween 20	MP Biomedicals	Cat# 11TWEEN201, CAS ID: 9005-64-5
Tween 40	Merck	Cat# P1504; CAS ID: 9005-66-7
EDTA	Millipore	Cat# 108421; CAS ID: 6381-92-6
EGTA	Merck	Cat# E3889; CAS ID 67-42-5
NaCl	Fischer Scientific	Cat# S/3160; CAS ID: 7647-14-5
MgCl2	Merck	Cat# M8266; CAS ID: 7786-30-3
LiCl	Merck	Cat# L4408; CAS ID: 7447-41-8
N-Lauroylsarcosine sodium salt solution 20%	Merck	Cat# L7414; CAS ID: 137-16-6
Formaldehyde	Merck	Cat# 25,254-9; CAS ID: 50-00-0
cOmplete™ Protease Inhibitor Cocktail, EDTA-free	Merck	Cat# COEDTAF-RO
Magna ChIP™ Protein A+G Magnetic Beads	Millipore	Cat# 16-663
Glycine Solution (10X)	Cell Signalling Technology	Cat# 7005S
ChIP Elution Buffer (2X)	Cell Signalling Technology	Cat# 7009S
5 M NaCl	Cell Signalling Technology	Cat# 7010S
Proteinase K	Thermo Fischer Scientific	Cat# EO0491
RNAse A	Thermo Fischer Scientific	Cat# EN0531
Tagmentation enzyme	Illumina	Cat# 15027916
Tagment buffer	Illumina
KAPA HiFi HotStart Ready Mix	KAPA Biosystems	Cat# KK2602
Agencourt AMPure XP beads	Fischer Scientific	Cat# A63881
Resuspension buffer	Illumina	Cat# 15026770
Ethanol absolute	VWR	Cat# 20821.296, CAS ID: 64-17-5
Cell lysis buffer 10x	Cell signaling technology	Cat# 9803
Sodium Orthovanadate	Merck	Cat# 450243, CAS ID: 13721-39-6
cOmplete™ Protease Inhibitor Cocktail	Merck	Cat# CO-RO
Digitonin	Merck	Cat# D141; CAS ID: 11024-24-1
Formalin, 10%	Sigma-aldrich	Cat# F5554
Critical Commercial Assays
GoScript™ Reverse Transcriptase kit	Promega	Cat# A5001
Maxwell® 16 LEV simplyRNA Purification Kit	Promega	Cat# AS1270
GoTaq® qPCR Master Mix	Promega	Cat# A6001
MinElute PCR purification kit	Qiagen	Cat# 28006
ATPlite luminescence system	Perkin Elmer	Cat# 6016949
Easysep Mouse hematopoietic progenitor cell isolation kit	Stem Cell Technologies	Cat# 19856
Western lighting chemiluminescence reagent plus	Perkin Elmer	Cat# NEL105
ImmPRESS HRP Anti-Goat IgG (Peroxidase) Polymer Detection Kit, made in Horse	Maravai Life Sciences	Cat# MP-7405
EnVision+ System	Agilent	Cat# K400011-2
Deposited Data
Raw and analyzed data	This paper	GSE102209
Human reference genome GRCh37/hg19	Genome Reference Consortium	https://www.ncbi.nlm.nih.gov/grc/human
Mouse reference genome GRCm38/mm10	Genome Reference Consortium	https://www.ncbi.nlm.nih.gov/grc/mouse
Experimental Models: Cell Lines
ALL-SIL	DSMZ	Cat# ACC-511, RRID:CVCL_1805
PEER	DSMZ	Cat# ACC 6, RRID:CVCL_1913
Experimental Models: Organisms/Strains
Mouse: C57BL/6	The Jackson lab	Cat# 000664
Mouse: C57BL/6 LSL-NA	GenOway	N/A
Mouse: C57BL/6 NA	This paper	N/A
Mouse: C57BL/6 NA+TLX1	This paper	N/A
Mouse: C57BL/6 TLX1	De Keersmaecker et al., 2010
Mouse: C57/BL/6 CD4-Cre	The Jackson lab	Cat# 017336
Mouse: C57/BL/6 CD2-Cre	Vacchio et al., 2014, The Jackson lab	Cat# 027406
Mouse: C57/BL/6 CD19-Cre	The Jackson lab	Cat# 018958
Oligonucleotides
Primers for genotyping	This paper	See Table S2
Primers for qRT-PCR	This paper	See Table S3
Negative control antisense LNA gapmer	Qiagen	Cat# LG00000002
TLX1 antisense LNA gapmer	Qiagen	Cat# 339511
STAT5A antisense LNA gapmer	Qiagen	Cat# 339511
STAT5A antisense LNA gapmer	Qiagen	Cat# 339511
STAT5B antisense LNA gapmer	Qiagen	Cat# 339511
STAT5B antisense LNA gapmer	Qiagen	Cat# 339511
Silencer™ Select Negative Control No. 1 siRNA	Thermo Fischer Scientific	Cat# 4390843
Silencer™ Select s9129 MYC siRNA	Thermo Fischer Scientific	Cat# 4392420
MYC siRNA sc-29226	Santa Cruz Biotechnology	Cat# sc-29226
Recombinant DNA
MSCV-EML1-ABL1-IRES-GFP	De Keersmaecker et al., 2005
MSCV-STAT5 N642H-IRES-GFP	Bandapalli et al., 2014
MSCV-TLX1-IRES-mCherry	This paper
Software and Algorithms
FlowJo	FlowJo, LLC	www.flowjo.com
GraphPad Prism	Graphpad software	www.graphpad.com
qBase+	Biogazelle	www.qbaseplus.com
CLC Main workbench	Qiagen Bioinformatics	www.qiagenbioinformatics.com/products/clc-main-workbench/
Integrative Genomics Viewer (IGV)	Broad Institute	software.broadinstitute.org/software/igv/
Bowtie2	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
SAMtools	Li et al., 2009	http://samtools.sourceforge.net/
MACS2	Zhang et al., 2008	https://github.com/taoliu/MACS
deeptools	Ramírez et al., 2016	http://deeptools.readthedocs.io/en/latest/
RSAT peak-motifs	Thomas-Chollier et al., 2012	http://pedagogix-tagc.univ-mrs.fr/rsat/RSAT_portal.html
fastq-mcf	Ea-utils	https://github.com/ExpressionAnalysis/ea-utils/blob/wiki/FastqMcf.md
FastQC	Babraham Bioinformatics	https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
Tophat2	Kim et al., 2013	https://ccb.jhu.edu/software/tophat/index.shtml
HTSeq	Anders et al., 2015	http://htseq.readthedocs.io/en/release_0.9.1/
DESeq2	Love et al., 2014	http://bioconductor.org/packages/release/bioc/html/DESeq2.html
SynergyFinder	Ianevski et al., 2017	https://synergyfinder.fimm.fi