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. Author manuscript; available in PMC: 2019 Sep 14.
Published in final edited form as: J Mol Biol. 2018 Jun 18;430(18 Pt A):2951–2973. doi: 10.1016/j.jmb.2018.06.017

Figure 3. Correction of class II CFTR variants by siRNA knockdown of target proteins.

Figure 3

A. Immunoblot analysis of CFTR and tubulin from a lysate prepared from CFBE41o- null cells transfected with the indicated siRNA and transduced with the indicated CFTR variant. B–E. Bar graph of the amount of band C detected by quantitation of the immunoblot shown in panel A for F508del-CFTR (B), G85E-CFTR (C), R560T-CFTR (D) and N1303K-CFTR (E). The data shown in panels B–E represents the percentage of band C relative to band B in the control siRNA condition (siScr). The data for the siScr represents n = 2 and the data for the siRNA knockdown for the CFTR variants are a single replicate (n = 1).

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