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. Author manuscript; available in PMC: 2019 Sep 14.
Published in final edited form as: J Mol Biol. 2018 Jun 18;430(18 Pt A):2951–2973. doi: 10.1016/j.jmb.2018.06.017

Figure 5. Vx770 restores a more WT-like PIPs to G551D-CFTR.

Figure 5

A. (Upper) Immunoblot analysis of CFTR and tubulin from a lysate prepare from CFBE41o- null cells transduced with the GFP, WT-CFTR or G551D-CFTR, the latter of which was treated with DMSO or 10 µM Vx770 for 24h. (Lower) Immunoblot analysis of CFTR immunoprecipitation from samples shown in panel A (upper). B. Heat map of the Jaccard similarity indices for the indicated pairwise comparisons. C. Heat map of bait normalized protein recovery in the immunoprecipitation of the indicated CFTR variant and treatment condition. The data is presented as a log2 of the additive peptide intensity normalized to the additive CFTR peptide intensity and sorted from most to least abundant based on recovery with WT-CFTR. D. Bar graph of the fold change in the binding affinity of the indicated proteins recovered in both the G551D versus WT and G551D+DMSO versus G551D+Vx770 differential PIPs. The data is shown as a log10 folding change of the total intensity for the indicated proteins with G551D relative to WT-CFTR (black) or Vx770 treated G551D (white).

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