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. Author manuscript; available in PMC: 2019 Sep 14.
Published in final edited form as: J Mol Biol. 2018 Jun 18;430(18 Pt A):2951–2973. doi: 10.1016/j.jmb.2018.06.017

Figure 6. Vx809 restores a more WT-like PIPs to F508del-CFTR.

Figure 6

A. CFTR immunoblot analysis of CFTR immunoprecipitation samples from CFBE41o- cell lysates virally transduced with WT- and G551D-CFTR treated with DMSO or 3µM Vx809 for 24h. B. Heat map of the Jaccard similarity indices for the indicated pairwise comparisons. C. Heat map of bait normalized protein recovery in the immunoprecipitation of the indicated CFTR variant and treatment condition. The data is presented as a log2 of the additive peptide intensity normalized to the additive CFTR peptide intensity and sorted from most to least abundant based on recovery with WT-CFTR. D. Bar graph depicting the percentage of YFP-H148Q/I152L quenching seen in CFBE41o- cells expressing F508del-CFTR in response to the siRNA-mediated silencing of the indicated protein whose binding affinity were statistically significantly altered by the treatment with Vx809.

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