The presence of hepatic steatosis is associated with increased expression of necroptosis markers following I/R surgery. [A] Hepatic gene expression of RIPK3, RIPK1, and MLKL in mice fed LF, HTF-C, or Western diet following sham or I/R surgery. [B] Western blots showing RIPK3 protein abundance and phosphorylation of the RIPK target MLKL in mice fed LF, HTF-C, or Western diet 24 h after sham or I/R surgery. Values are mean ± standard error of the mean. *p<0.05 LF vs HTF-C. #p<0.05 LF vs Western. †p<0.05 HTF-C vs Western. Abbreviations: AU, arbitrary units.