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. Author manuscript; available in PMC: 2019 Aug 16.
Published in final edited form as: Mol Cell. 2018 Jul 26;71(4):621–628.e4. doi: 10.1016/j.molcel.2018.06.030

Figure 2.

Figure 2

FANCA promotes strand exchange. Titration of the purified FANCA-WT and F1263Δ mutant (10, 20, 30, and 40 nM calculated as monomers) on (A) a pre-annealed splayed arm structure (Right panel, quantification of the SE activity; Experiments were independently repeated 3 times; Error bar, standard deviation); (B) a dsDNA structure with partial 5′-end resection of one strand and a short blunt-ended dsDNA with full homology to the uncovered flap of the invading DNA structure; and (C) a dsDNA structure with partial 3′-end resection of one strand and a short blunt-ended dsDNA with full homology to the uncovered flap of the invading DNA structure. Quantification of SE activity is represented by % product below each gel. (D) Titration of the purified FANCA-WT, FANCA-F1263Δ mutant, and RAD52 (10, 20, 30, 40, and 50 nM) in a D-loop formation assay. Red asterisks (*) indicate 32P labeling.