Figure 6.

Adherence of E. faecium AUS0004 wild type and its isogenic mutant strains, the triple mutant ∆EFAU004_01059-01150-494 and the single ΔEFAU004_01209 mutant, and the complemented strain Δ1209:1209 to kidney epithelial VERO cells after two and twenty-four hours. Cells were infected with an MOI of 100:1. Adherent bacteria were collected after 2 and 24 hours and enumerated by plating serial dilutions on BHI agar plates and counting CFU. The bars indicate standard deviations. The assay was performed in triplicate and repeated twice. The analysis was performed by two-way ANOVA. p values < 0.01 were considered significant. ^**p = 0.001.