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. 2018 Aug 17;8:12412. doi: 10.1038/s41598-018-30882-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence microscopy of the enterococcal strains labeled with anti-1209 serum coupled with fluorescein isothiocyanate (FITC) (green); cells are stained with Evans blue. (A) A1 ΔEFAU004_01209. No signal is detected. A2 relative phase channel (B) B1 Anti-CcpA serum on total protein extracts of the E. faecalis JH2-2 strain; no immunofluorescence signal is detected due to the cytoplasmic localization of the CcpA protein. B2 Relative phase channel (C) C1 AUS0004 wild-type strain shows green fluorescence, indicating a possible surface localization of the EFAU004_1209 protein. C2 relative phase channel (D) D1 Triple mutant Δ1050-1150-494. D2 Relative phase channel.