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. 2018 Aug 17;8:12302. doi: 10.1038/s41598-018-30696-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of Planocera multitentaculata in the intestinal contents of young wild Takifugu niphobles individuals (a) and TTX content (mg/individuals) in the pufferfish individuals (b). The arrow indicates PCR products specific to P. multitentaculata. Lane M: molecular weight marker; lane A: anterior part of the intestine; lane C: middle part of the intestine; lane P, posterior part of the intestine; lane PC, positive control (P. multitentaculata). P. multitentaculata-specific band is present in No. 3 and 5 individuals. Full-length gel is included in a Supplementary Information file (Fig. S4).