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. 2018 Aug 17;8:12380. doi: 10.1038/s41598-018-30880-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of tertbutylhydroquinone (A, “tBHQ”), sulforaphane (B, “SFN”), hydrogen peroxide (C), and dimethyl sulfoxide (D, “DMSO”) on luminescence (bars) and viability (lines) measured in the zebrafish cell line ZF4. Luminescence corresponds to quantitative Nrf2 activation measured via dual reporter gene assay. Viability corresponds to measured absorbance of formazan product via MTS-assay. Each bar and point respectively represent the mean (experimental units n = 3–4; observational units N = 10–16) including SD. Asterisks indicate significance tested in a two-way ANOVA mixed model with Dunett’s post-hoc test (*p < 0.05, **p < 0.01, ***p < 0.001; grey = viability; black = luminescence).