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. 2018 Aug 17;8:12354. doi: 10.1038/s41598-018-30799-7

Figure 2.

Figure 2

The effect of SHH treatment on HEMF GLI1 expression and on BMP4 expression and BMP4 protein secretion. (a) GLI1 and BMP4 expression were evaluated by qRT-PCR in HEMFs treated with increasing doses of SHH. Relative change of the target gene is shown by the 2∆∆ (delta) CT method. HEMF expression of GLI1 and BMP4 increase in response to treatment with SHH. Results represent the means ± SEM of three individual experiments performed in HEMFs. *P ≤ 0.04; **P ≤ 0.02; NS, non-significant vs. no treatment for each gene per ANOVA for multiple comparisons (b) HEMF secretion of BMP4 in response to treatment with SHH was evaluated by ELISA. BMP4 secretion increases in response to treatment with SHH. Results represent the means ± SEM of three individual experiments performed in HEMFs. *P ≤ 0.02; **P ≤ 0.01; ***P ≤ 0.001 versus no treatment per ANOVA for multiple comparisons.