Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 17;8:12354. doi: 10.1038/s41598-018-30799-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HEMF Gremlin1 secretion and BMP4 detection. (a) HEMFs secretion of Gremlin (GREM)1 was evaluated by ELISA performed on conditioned media of untreated HEMFs. Results represent the means ± SEM of three individual experiments performed in previously described primary cultures (HEMF 1, 2) and a GFP-hTERT transfected immortalized HEMF cell line (HEMF 5). (b) Detection of BMP4 in HEMF conditioned media was with ELISA after the addition of GREM1 nAb. Detection of BMP4 increased with the addition of GREM1 nAb to HEMF conditioned media, both in the presence and absence of HEMF treatment with SHH. Results represent the means ± SEM of three individual experiments performed in HEMFs. *P ≤ 0.02; **P ≤ 0.01; NS, non-significant with ANOVA for multiple comparisons.