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. 2018 Aug 17;8:12354. doi: 10.1038/s41598-018-30799-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differentiation of epithelial cells in 3D organotypic ALI culture in the presence of conditioned HEMF media and GREM1 nAb. Representative confocal images of immunostaining performed for basal cell marker CK14 (rhodamine; red) and suprabasal marker CK4 (Cy2; green) in squamous epithelium cultured with control serum-free myofibroblast media (SFMM), conditioned SFMM (cSFMM) and cSFMM plus GREM1 nAb (n = 3 each condition) are shown. Magnification 63X. Expression of basal marker CK14 is more prominent with cSFMM compared to control. Addition of GREM1 nAb decreases intensity of immunostaining for basal marker CK14 and increases intensity of suprabasal marker CK4.