Fig. 2.

5′-End extensions of the crRNA enhance the gene editing of Cpf1 in HEK cells. a Sequence of the GFP protospacer that is targeted by both AsCpf1 and SpCas9. b Electroporation of SpCas9 and AsCfp1 RNP targeting the GFP-matched site demonstrate that SpCas9 can knock out genes more efficiently than Cpf1. Mean ± SE, n = 4. *p < 0.05 by Student’s t test. c 5′ Sequence extension of crRNA increases Cpf1 gene editing in GFP-HEK cells. Cpf1 RNP+ with various 5′ extended crRNAs were delivered to GFP-HEK cells using electroporation. Four nucleotide to twenty-five nucleotide extension significantly increased editing efficiency. Mean ± SE, n = 3. **p < 0.01 by Student’s t test compared to crRNA control