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. 2018 Aug 17;8:12337. doi: 10.1038/s41598-018-30886-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nuclear expression of novex-3 in hypoxic fCMs is repressed by exposure to higher O₂. (a) Immunofluorescence staining for novex-3 (green) observed in phalloidin (red) and DAPI (blue) in E17 fCMs cultured under different O₂ conditions. Scale bar = 20 µm. (b) Immunofluorescence staining for novex-3 (green, another e2 antibody was used; see Methods) observed in sarcomeric α-actinin (red, as a CM marker) and DAPI (blue) in E17 fCMs cultured under different O₂ conditions. Scale bar = 20 µm. (c) qPCR analysis of novex-3 mRNA expression in fCMs cultured under different O₂ conditions. n = 3 independent experiments. **P < 0.01 compared to 3% O₂. Error bar = SEM. (d) Immunoblot analysis for novex-3 protein in nuclear fraction from E17 fCMs cultured under different O₂ conditions. Histone H3 was included as a loading control.