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. 2018 Aug 17;7:149. doi: 10.1038/s41426-018-0148-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Each target gene in the shuttle plasmid pEDL17 was expressed with a His tag from a tetracycline-inducible promoter in LVS (open bar) or isogenic ∆lon mutant (filled bar). Proteins were detected by immunoblotting (a–h) and quantified by Image Lab (i) as in Fig. 1. Abundance ratio of each protein between LVS and the ∆lon mutant is indicated at the top of relevant bars. Each bars represents the mean value ± SEM (n = 3). The protein encoded by endogenous (chromosomal) FTL1017 was detected with an antiserum as a loading control