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. 2018 Aug 17;7:149. doi: 10.1038/s41426-018-0148-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Stability of the fLon substrates was detected in the presence of fLon (a), fLon^S682A (b), eLon (c), or hLon (d) in E. coli ER2566. Each set of the lon and target genes were cloned in two compatible plasmids behind either arabinose (for Lon)- or IPTG (for substrate)-inducible promoter. Protein detection and quantification were carried out as in Fig. 1