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. 2018 Aug 17;8:12315. doi: 10.1038/s41598-018-30947-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of Ad capsid protein-specific sera isolated from Ad capsid protein-expressing plasmid-immunized mice. (A) ELISA analysis using Ad capsid protein-specific sera. Ad-null was solubilized by 0.1% Triton X-100 and immobilized on a plate. Anti-Ad capsid protein sera were diluted and added to the well. The gray shaded boxes indicate background levels. The data are expressed as the mean ± S.D. (n = 4). n.s., not significant, *p < 0.05, **p < 0.01, ***p < 0.001, compared with naïve sera. (B) western blotting analysis using Ad capsid protein-specific sera. Ad-null was denatured at 98 °C for 5 min and loaded on SDS-PAGE gels according to the manufacturer’s protocol. Western blot analysis was carried out using anti-Ad capsid protein sera. Each image was captured under different exposure times. Representative images from three independent experiments using different mouse serum batches are shown.