Table 1.
A Comparison of the Biophysical Properties of WT-ISCU and Proposed Suppressor Variants. Standard deviations were determined from experimental replicates of n = 3.
| Variant | Additive | [S2−]/[NFS1]/min Without FXN |
[S2−]/[NFS1]/min With FXN |
ZnCl2 IC50 (μM) | Tm (°C) |
Kd (nM) (BLI of SDA-U) |
|---|---|---|---|---|---|---|
| WT | EDTA | 1.75 ± 0.16 | 8.14 ± 0.42 | NA | 33.2 ± 0.6 | 87.8 ± 7.0 |
| ZnCl2 | 0.21 ± 0.02 | 6.42 ± 0.16 | 0.90 ± 0.06 | 65.7 ± 0.2 | 160 ± 26 | |
| M140I | EDTA | 1.51 ± 0.18 | 4.43 ± 0.20 | NA | 34.2 ± 0.7 | 97.3 ± 8.7 |
| ZnCl2 | 0.25 ± 0.10 | 2.11 ± 0.09 | 1.71 ± 0.14 | 64.7 ± 0.4 | 194 ± 31 | |
| M140L | EDTA | 1.92 ± 0.14 | 6.77 ± 0.33 | NA | 30.9 ± 0.2 | 110 ± 11 |
| ZnCl2 | 0.27 ± 0.11 | 4.02 ± 0.18 | 0.52 ± 0.09 | 63.5 ± 0.2 | 181 ± 28 | |
| M140V | EDTA | 1.83 ± 0.05 | 5.69 ± 0.26 | NA | 34.3 ± 0.5 | 85.5 ± 6.8 |
| ZnCl2 | 0.32 ± 0.15 | 2.67 ± 0.17 | 0.90 ± 0.07 | 64.6 ± 0.3 | 218 ± 30 |