Figure 2.

Seven kinase inhibitors enhance dual AAV serotype 2 (AAV2) transduction in vitro at higher levels than PI. Representative Western blot (WB) analysis (n ≥ 2 replicates) of lysates from HEK293 cells infected with dual AAV2 vectors expressing eGFP and incubated with the drugs at the indicated concentrations. For each sample, 50 μg of protein were loaded. α-eGFP, WB with anti-eGFP antibody; α-tub, WB with anti-tubulin antibody, used as loading control; Neg, not treated cells; dual AAV, dual AAV2 expressing eGFP; DMSO, dimethyl sulfoxide (baseline control, the drug solvent).