Figure 4.

The kinase inhibitors enhancement of dual AAV transduction occurs with different transgenes and AAV serotypes. (A and B) RNA (dark gray; n = 4) and protein (light gray; n ≥ 4) quantification measured by reverse transcription quantitative polymerase chain reaction and WB analysis, respectively, in lysates from HEK293 cells infected with dual AAV2 vectors expressing either ABCA4 (A) or MYO7A (B) and incubated with the various drugs. (C) Cytofluorimetric analysis (n = 3) of eGFP expression in HEK293 cells infected with various dual AAV-eGFP serotypes and incubated with F13 5 μM (dark gray), H17 3 μM (light gray), and K17 0.3 μM (medium gray). Values, normalized to the corresponding DMSO sample, are presented as the mean ± SE. *p < 0.05.