Figure 7.

F13 enhances dual AAV8-mediated transduction of the mouse retina. Representative WB image (A) and quantification (B) of retinal lysates of C57BL/6 eyes co-injected sub-retinally with kinase inhibitors at the indicated concentrations, and dual AAV8 expressing eGFP-3xflag under the control of the PR-specific GRK1 promoter (Dual-GRK1-eGFP-3xflag) or not injected (Neg). For each sample, 65 μg of protein was loaded. Ref, lysate from HEK293 cells transfected with a plasmid expressing eGFP-3xflag used as a reference; α-3xflag, WB with anti-3xflag antibody; α-Calnexin, WB with anti-Calnexin antibody used as loading control; In (B), the values (n = 7), normalized to a standard sample present in every WB, are presented as the mean ± SE. *p < 0.05.