Fig. 1.

HK2 is dispensable for T cell glycolysis. Naïve CD4+ and CD8+ T cells were enriched from splenocytes isolated from adult WT and T-Hk2^−/− mice. T cells were activated in vitro with anti-CD3/28-coated beads for 24–72 h. a RNA expression of HK isoforms in CD4+ and CD8+ T cells after 24 h stimulation, compared to naïve unstimulated cells. Expression relative to β-actin and Rpl-19. Biological replicates, n = 4–5 (as indicated by individual data points) from at least two independent experiments b Western blot for protein expression of HK2 in muscle tissue or CD4+ T cells after 24 h stimulation. Representative of four independent experiments. c–f CD4+ or CD8+ T cells stimulated for 72 h and assayed for c total HK activity, d 2-DG uptake, e extracellular acidification rate (ECAR), and f oxygen consumption rate (OCR). c–f At least two independent experiments performed, biological replicates, n = 3–7 as indicated by individual data points. In all panels unless otherwise noted, error bars represent mean ± SEM, one-way or two-way ANOVA with Sidak multiple comparisons, *p < 0.05; **p < 0.01. NS = not significant