Fig. 3.

Inflammatory cytokines have opposite effects on the migratory properties of growing and dormant cells. a Cytokines decrease the motility of growing cells. Fifty thousand MCF-7 cells were incubated on fibronectin-coated 10 cm plates. The following day (day 0), DMEM/10% FCS without or with recombinant human FGF-2, IL-6, IL-8 or TGFβ1 were added to the plates to achieve a final concentration of 10 ng/ml. On day 6, cells were detached into single cell suspension by trypsin and 100,000 cells were added to the CIM-plate wells. Arrow indicates decreased motility of cytokine-treated growing cells compared to that of growing cells. b Cytokines increase the motility of dormant cells. Fifty thousand MCF-7 cells were incubated on fibronectin-coated 10 cm plates and DMEM/10% FCS and FGF-2 was added the following day. Six days later, DMEM/10% FCS without or with recombinant human IL-6, IL-8 or TGFβ1 were added to the cultures. On day 12, cells were detached into single cell suspension by trypsin and 100,000 cells were added to each well of the CIM-plate. Trypsin-dispersed day 6 dormant cells were used as negative controls. Arrow indicates increased motility of cytokine-treated dormant cells compared to that of dormant cells. Results represent the average of triplicate wells