Table 3.

Variants of Lactococcus lactis FM03P with different plasmids contents that were obtained in this study

L. lactis variant	Cured plasmids	Plasmid content	Isolated from
FM03P	–	pLd1,2,3,4,5,6,7,8,9,10,11,12	Samsø cheese
3	pLd4,5,10	pLd1,2,3,6,7,8,9,11,12	Protoplast-induced curing
5	pLd4,10,11	pLd1,2,3,5,6,7,8,9,12	Protoplast-induced curing
47	pLd8,11	pLd1,2,3,4,5,6,7,9,10,12	Overnight culture
48	pLd2,4,5,6,7	pLd1,3,8,9,10,11,12	Chemostat culture
49	pLd2,3,4,5,6,7	pLd1,8,9,10,11,12	Protoplast-induced curing
50	pLd4,5,6,7	pLd1,2,3,8,9,10,11,12	Protoplast-induced curing
51	pLd4,5,6,7,8,9,11,12	pLd1,2,3,10	Protoplast-induced curing
63	pLd3,4,8,11,12	pLd1,2,5,6,7,9,10	Protoplast-induced curing
FM03-V1	pLd7	pLd1,2,3,4,5,6,8,9,10,11,12	Chemostat culture

Variants 47, 48 and FM03-V1 were isolated by plating on LM17 agar plates