Table 4.
Primer sequences used to detect plasmids in L. lactis FM03P
| Target | Sequence (5′-- > 3′) | Amplicon | Tm (°C) | |
|---|---|---|---|---|
| Chromosome | Fw | TTAATTCAACCTGGAGACACAGTCTTAG | 254 | 65.2 |
| Rv | CTATCAGCGATTTCACGGAACTTAG | 65.6 | ||
| pLd1 | Fw | GCATTGACGGCTGTTGTAAT | 209 | 62.5 |
| Rv | AGCAGATTCCCGAGGATAAC | 62.0 | ||
| pLd2 | Fw | AATGGGCCGAAGGTTCTATT | 285 | 63.4 |
| Rv | CAGGAACCGATTCTCCTGTTA | 62.9 | ||
| pLd3 | Fw | CCTCTCGCGTTCCTTGATA | 417 | 62.9 |
| Rv | CCACGTAAGGGCGATTTAGT | 62.7 | ||
| pLd4 | Fw | GCGGTAACAACATCCGTATC | 508 | 61.8 |
| Rv | AGTCAGCCCAAGCGACTAAT | 62.7 | ||
| pLd5 | Fw | AAATACAAGTGTTGAAGGCGTTG | 589 | 63.8 |
| Rv | ACCTTTGTTCTCCAATTTCAGC | 62.2 | ||
| pLd6 | Fw | TAAGTGCAACTAAAAGAAATAATAAAGTGCAA | 200 | 65.1 |
| Rv | TTGCTGATGATTGTACCAGCTAAAAC | 65.8 | ||
| pLd7 | Fw | TGGGCATCTAGATAATCTGACGACATCTGT | 596 | 71.3 |
| Rv | CGACATTGACTCCCCAAAAACCAAAAATGA | 75.3 | ||
| pLd8 | Fw | CCCAGTTGATTTAGAATTAGCTGAAGAATA | 588 | 65.8 |
| Rv | AGATAGGTTGCATCCAAGATAAATTTGTTA | 65.9 | ||
| pLd9 | Fw | TAGTCGCTGGCAAATTTTACAATCA | 323 | 67.0 |
| Rv | CTTTGGGGGTTGCTTTAGAATCAAT | 67.5 | ||
| pLd10 | Fw | ACGCTTGAACCCCATCTTGG | 255 | 68.3 |
| Rv | TCGTCCCAAACGGTTTACCC | 67.8 | ||
| pLd11 | Fw | TTCAATGAATGGCTCGGAAGAA | 388 | 67.6 |
| Rv | TTTCGGCACAGGAGCAACAT | 67.9 | ||
| pLd12 | Fw | GAAAACTAATCTAGTACAATCATCAGCAAACTT | 277 | 65.1 |
| Rv | TTTTTACGAGTTATATTGTTTCTAGTCAGATTCTT | 64.7 | ||
Primers were used to determine the plasmid content of different variants and to discriminate variants in competition experiments