Fig. 7.

Dual-luciferase reporter assay. a HepG2/5-FU and SMMC7721/5-FU cells were co-transfected with plasmids overexpressing wild-type or mutant KRAL and luciferase reporter vector containing either Keap1 3’UTR or empty vector (pmirGLO). Relative luciferase activity was presented as the relative ratio of firefly luciferase activity to Renilla luciferase activity. Data are expressed as the mean ± SD; columns: normalized mean of three independent experiments,*p < 0.05 vs the pLV-control group or pLV-KRAL-mut group, **p < 0.01 vs the KRAL1 group or pLV-KRAL-mut group. b HepG2 or SMMC7721 cells were co-transfected with shRNA-KRAL or mutant KRAL plasmids and luciferase reporter vector containing either the Keap1 3’UTR or empty vector (pmirGLO). Relative luciferase activity was presented as the relative ratio of firefly luciferase activity to Renilla luciferase activity. Data are expressed as the mean ± SD; columns: mean of three independent experiments; *p < 0.05 vs the pLV-control group or pLV-KRAL-mut group, **p < 0.01 vs the shRNA-KRAL-1 group or pLV-KRAL-mut group