| Chr 12 enhancer gRNA 1 |
F |
5′-GTATATATTCATACAGGACGTTTT-3′ |
| R |
5′-GTCACTGTATGAATATATACCGGTG-3′ |
|
| Chr 12 enhancer gRNA 2 |
F |
5′-TCGCTGCACATTGACTCTAAGTTTT-3′ |
| R |
5′-TTAGAGTCAATGTGCAGCGACGGTG-3′ |
|
| Chr 12 enhancer gRNA 3 |
F |
5′-GGAGCTCGCTGCACATTGACGTTTT-3′ |
| R |
5′-GTCAATGTGCAGCGAGCTCCCGGTG-3′ |
|
| Donor_5′arm |
F |
5′-AGAACTAGTGGATCCCCCGGGCTGCAGGAATTCGATAT CAATTTCCCAAAAAGGAAAGGTATCTAACAAATTTATTCAC-3′ |
| R |
5′-GGGATAGGAGAGCTTAAAATCGAGCTCCAGGGTTTACT CC-3′ |
|
| Donor_targeting |
F |
5′-GGAGTAAACCCTGGAGCTCGATTTTAAGCTCTCCTATC CCATATATTTGCAATTATTTG-3′ |
| R |
5′-CCTGTCACTGTATGAATATAATAAAGCATATCTGTGTG GAGCAGAG-3′ |
|
| Donor_3′arm |
F |
5′-TCCACACAGATATGCTTTATTATATTCATACAGTGACA GGAATGACTGTCATAATAC-3′ |
| R |
5′-CCTCACTAAAGGGAACAAAAGCTGGGTACCGGGCCCCC CCGCATTTACTGTCAGTTTGTTTTACCAAAAAAATAATAAT AACTCA-3′ |
|
| PCR screen for human allele |
F |
5′-GGCCCTTAGCACTCTCTTTG-3′ |
| R |
5′-CAGCCGTCCCTGAAAATAGA-3′ |
|
| PCR targeting 5′ end |
F |
5′-TGATTTCCCAAAAAGGAAAGG-3′ |
| R |
5′-GCGAGCTCCAGGGTTTACT-3′ |
|
| PCR targeting 3′ end |
F |
5′-TTGATTGTCCTCTGCACCAC-3′ |
| R |
5′-AGGGGAAAGGAAAGACTCCA-3′ |
|
| Cleav 5′ |
F |
5′-AGCTCGCTGCACATTGACT-3′ |
| R |
5′-CCAATCACATTTTGCGACAC-3′ |
|
| Cleav 3′ |
F |
5′-TGAGGGGCTACCCATGACAT-3′ |
| R |
5′-TGCGCATCCGTTTTGTTACA-3′ |
|
| gRNA3 off-target 1 F |
F |
5′-TCTGCTCCCTGAGTGCTGTA-3′ |
| R |
5′-AGAGAAGTGCTTGGCTTCCA-3′ |
|
| gRNA3 off-target 2 F |
F |
5′-CTCTGTAGCTCGGCAGGAAC-3′ |
| R |
5′-GCTGAGCTGGAAGGATGAAC-3′ |
|
| gRNA3 off-target 3 F |
F |
5′-TCGGAGAGGAGCTACCTGAA-3′ |
| R |
5′-CCGATCCACTCCTTACCTCA-3′ |
|
| gRNA3 off-target 4 F |
F |
5′-TACCGATCCACGTCCTTCAC-3′ |
| R |
5′-CTCAACACTCGGGGTCAAGT-3′ |
|
| gRNA3 off-target 5 F |
F |
5′-CAATTTGTAGCCCTGGCTGT-3′ |
| R |
5′-CGCCATAGCTGCTTTCTACC-3′ |
|
| gRNA5 off-target 1 F |
F |
5′-GGCTTCCTTCCCTGTAGGTC-3′ |
| R |
5′-GCAATAAAGGCAGAGCTTGG-3′ |
|
| gRNA5 off-target 3 F |
F |
5′-TCTTGGGGTTTCCTACCAGA-3′ |
| R |
5′-ACTACAGGGCCCATGAGCTA-3′ |
|
| gRNA5 off-target 4 F |
F |
5′-TGCATGCCTGTTCATTAAGC-3′ |
| R |
5′-GGCAGATGGTGTCTGTAGTCC-3′ |
|
| gRNA5 off-target 5 F |
F |
5′-TCTGCATTTCTGTGGTCAGC-3′ |
| R |
5′-TTGTGAGTGAGGGTGTTGGA-3′ |
|
| Rx gRNA 5′ |
F |
5′-GGATTCGTCCCGGAGTACGAGTTTT-3′ |
| R |
5′- TGCACCTGCCGGGCTGCGCGCGGTG-3′ |
|
| Rx gRNA 3′ |
F |
5′-CGCGCAGCCCGGCAGGTGCAGTTTT-3′ |
| R |
5′-TCGTACTCCGGGACGAATCCCGGTG-3′ |
|
| Rx screening |
F |
5′-GTTTAGCTTTGGACAGACCG-3′ |
| R |
5′-GAGGTTCGGCAGTCACTGTG-3′ |
|
| Arl13b gRNA 5′ |
F |
5′-ATGGTCGGCCTTGATAATGCGTTTT-3′ |
| R |
5′-GCATTATCAAGGCCGACCATCGGTG-3′ |
|
| Arl13b gRNA 3′ |
F |
5′-TTATGGGTCATCACAACATAGTTTT-3′ |
| R |
5′-TATGTTGTGATGACCCATAACGGTG-3′ |
|
| Arl13b screening |
F |
5′- TGATACTTTCACGGGGATCG-3′ |
| R |
5′- TCCTGCTGATCAACAACTTCA-3′ |
