Skip to main content
. Author manuscript; available in PMC: 2019 Aug 20.
Published in final edited form as: Free Radic Biol Med. 2018 Jun 5;124:149–162. doi: 10.1016/j.freeradbiomed.2018.05.094

Figure 5. Incision of 8-oxodG is less efficient in mitochondria isolated from hOGG1S326C cells than hOGG1 cells.

Figure 5

A. Cells were grown in the presence of DMSO (0.1%) or rotenone (5 μM) for 24 hours and then harvested. Western blot demonstrates equal expression of hOGG1 protein in hOGG1S326C and hOGG1 cells and no hOGG1 expression in vector cells. COX IV expression is shown as a mitochondrial marker. B. Cell extracts were diluted to protein equal concentrations in reaction buffer and incubated with substrate shown in Figure 1A. Fragments were separated by denaturing electrophoresis and visualized using phosphoimaging. Graphs show quantitation of incised band relative to total radioactivity in lane. The percent incised was divided by the OGG1 content in each sample and normalized to activity of hOGG1wild type extracts in each experiment. Significance was determined by 2-way ANOVA. C. Cells were grown in the presence of DMSO (0.1%) or compound F (15 μM) and harvested after 24 hours. Incision of 8-oxodG was assessed as above. All experiments included three biological repeats. Significance was determined by 2-way ANOVA.