Immunohistochemistry for NLRP3 revealed staining in mouse (n = 14; A: adult, 3 months) rhesus macaque (n = 4; B: adult, 18 years), common marmoset (n = 7; C: adult, 11 years; G: new-born) and human testes (n = 8; D: adult, 48 years). Sertoli cells expressed NLRP3 in all samples examined. Expression in peritubular cells (arrows) of the seminiferous tubule was solely detected in human samples. Higher magnifications of adult marmoset (E) and human (F) sections confirmed these findings. Inlays: negative controls. Bars = 25 μm.