Hydrolysis of buffalo whey with Alcalase. Samples were hydrolyzed with 2% (v/v) Alcalase at 50 °C for up to 6 h and values of degree of hydrolysis (DH, triangle, %) were determined at the indicated intervals. The antioxidant activity of the hydrolysates was measured by ABTS·+ radical scavenging activity (diamond, %), iron chelating activity (plus sign, %), and reducing power (multiplication sign, Abs700nm,). Values are the mean ± standard deviations of three independent experiments. Data on the same line accompanied by different letters differ statistically from each other by the Tukey test (p < 0.05)