Table 1.

Primer sets for reverse transcription polymerase chain reaction.

Name	Sequence	Annealing temp (°C)	Size (bp)
GAPDH	F: GCC AAA AGG GTC ATC ATC TC	61	379
	R: ACG GAT ACA TTG GGG GTA GG
Oct4	F: CCC GGA AGA GAA AGC GAA CT	58	362
	R: GAC GGG AAC AGA GGG AAA GG
Nanog	F: AGG GTC TGC TAC TGA GAT GCT CTG	56	363
	R: CAA CCA CTG GTT TTT CTG CCA CCG
LIF	F: ATT GTG CCC TTA CTG CTG CT	61	583
	R: GCC TGG ACC ACC ACA CTT AT
CT-1	F: GAG GAA TAC GTG CAG CAA CA	57	389
	R: AGC ACC TTG GCT GAG AAG AT
OSM	F: CAC GGC TTC TAA GAA CAC TGC	59	547
	R: CGA TGG TAT CCC CAG AGA AA
CNTF	F: CTT TCG CAG AGC AAT CAC CT	61	579
	R: CCC CAT AAT GGC TCT CAT GT
BMP2	F: TCC ATC ACG AAG AAG CCG TG	58	465
	R: CCA AAA GTC ACT AGC AAT GGC
BMP4	F: AGG GCC AGC ACG TCA GAA TC	57	430
	R: ACC TTG TCA TAC TCA TCC AGG
CNTFRα	F: CTG TTT CCA CCG TGA CTC CT	59	802
	R: TGG GAC ACT GGT CAA GAA GA
Nodal	F: GCC AGA CAG AAG CCA ACT GTG	61	324
	R: TCA GAG GCA CCC ACA CTC CTC
GluR6	F: CTG CAG CAC AGA GAG GAA CCA	60	488
	R: ATA ACT TCC TCC ATG TGC CTC AC
Brachyury	F: GCT GAG ACT TGT AAC AAC CG	55	266
	R: GCA AAG GAC TCT GAT TAA CTG C
TTR	F: CCG TTC CAT GAA TTC GCG GAT	60	240
	R: TTC ACG GCA TCT TCC TGA GC
AFP	F: TTG CCT CCA CGT GCT GCC AGC	61	341
	R: CGC CAG CTG CTC CTC TGT CAG