Table 1.
Percentage of live, apoptotic, and dead A549 cells expressed as means ± SD and analyzed by Annexin V and Dead Cell assay kit using Muse Cell Analyzer.
| Live | Total apoptotic∗ | Dead | |
|---|---|---|---|
| 8 h of incubation | |||
| Untreated control | 88.42 ± 1.45 | 10.72 ± 0.99 | 0.87 ± 0.29 |
| Positive control H2O2 (5 mM) | 67.07 ± 2.60 | 27.98 ± 4.84 | 4.93 ± 2.30 |
| OMVs (20 μg/ml) | 82.21 ± 0.35 | 17.70 ± 0.28 | 0.28 ± 0.18 |
| OMVs (20 μg/ml) + SP (10−8 M) | 81.64 ± 5.56 | 17.83 ± 5.06 | 0.5 ± 0.49 |
| OMVs (20 μg/ml) + CGRP (10−8 M) | 84.78 ± 0.81 | 14.90 ± 0.78 | 0.23 ± 0.18 |
| OMVs (100 μg/ml) | 78.28 ± 1.31 | 20.23 ± 3.01 | 0.38 ± 0.39 |
| OMVs (100 μg/ml) + SP (10−8 M) | 82.18 ± 1.45 | 17.40 ± 1.48 | 0.43 ± 0.04 |
| OMVs (100 μg/ml) + CGRP (10−8 M) | 86.00 ± 2.33 | 13.40 ± 2.19 | 0.6 ± 0.14 |
| SP (10−8 M) | ND | ND | ND |
| CGRP (10−8 M) | ND | ND | ND |
| 24 h of incubation | |||
| Untreated control | 86.25 ± 2.14 | 12.88 ± 1.8 | 0.87 ± 0.55 |
| Positive control H2O2 (5 mM) | 38.51 ± 0.35 | 59.99 ± 0.98 | 1.49 ± 1.34 |
| OMVs (20 μg/ml) | 83.98 ± 2.86 | 15.00 ± 3.04 | 1.03 ± 0.18 |
| OMVs (20 μg/ml) + SP (10−8 M) | 90.08 ± 0.60 | 9.10 ± 0.21 | 0.83 ± 0.39 |
| OMVs (20 μg/ml) + CGRP (10−8 M) | 83.33 ± 2.72 | 15.83 ± 2.72 | 0.85 ± 0.01 |
| OMVs (100 μg/ml) | 68.25 ± 2.18 | 30.98 ± 2.20 | 0.70 ± 0.13 |
| OMVs (100 μg/ml) + SP (10−8 M) | 80.05 ± 0.07 | 17.80 ± 1.98 | 2.15 ± 2.05 |
| OMVs (100 μg/ml) + CGRP (10−8 M) | 83.68 ± 3.08 | 14.85 ± 1.34 | 1.48 ± 1.73 |
| SP (10−8 M) | 82.23 ± 0.46 | 15.30 ± 1.70 | 2.48 ± 2.16 |
| CGRP (10−8 M) | 89.33 ± 1.52 | 9.43 ± 2.05 | 1.23 ± 0.53 |
∗Total apoptotic refers to early apoptotic and late apoptotic cells. ND: not determined.