Table 2.

Characteristics of Multi-specie Biofilms of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, Cell surface hydrophobicity, Planktonic population and SCVs or persister cells population at pre and post biofilm stage

Combinations	S #	Biofilm OD578a	Cell surface hydrophobicity			Pre-biofilm population after 18 hr incubation			Biofilm population after 48 hr of incubation			SCVs or persister cell population of biofilms
			18 hr	24 hr	48 hr	E. coli	S. aureus	P. aeruginosa	E. coli	S. aureus	P. aeruginosa	E. coli	S. aureus	P. aeruginosa
Biofilm positive isolates in multi-specie biofilms	1	0.93	0.45	0.68	0.77	1x105	1x104	1x102	1x102	<10CFU	1x103	1x101	<10CFU	1x102
	2	0.92	0.33	0.66	0.71	1x105	1x104	1x102	1x102	<10CFU	1x103	1x101	<10CFU	1x102
	3	0.89	0.41	0.73	0.82	1x105	1x104	1x103	1x102	1x103	1x103	1x101	<10CFU	1x102
	4	0.97	0.44	0.77	0.87	1x105	1x104	1x103	<10CFU	1x103	1x103	<10CFU	1x101	1x102
	5	0.99	0.22	0.71	0.89	1x104	1x104	1x103	1x102	<10CFU	1x103	1x101	<10CFU	1x102
	6	1.01	0.24	0.73	0.94	1x105	1x104	1x103	1x102	<10CFU	1x103	<10CFU	<10CFU	1x102
	7	1.03	0.26	0.71	0.91	1x105	1x105	1x103	<1CFU	1x103	1x103	<10CFU	<10CFU	1x102
	8	0.92	0.34	0.67	0.77	1x104	1x105	1x103	1x103	<10CFU	1x103	<10CFU	<10CFU	1x102
	9	1.04	0.27	0.69	0.93	1x105	1x104	1x103	1x103	<10CFU	1x103	<10CFU	<10CFU	1x102
Biofilm Negative Isolates in multi-specie biofilms	1	BND	0.22	0.68	0.51	1x105	1x104	1x104	BND	BND	BND	BND	BND	BND
	2	BND	0.19	0.66	0.52	1x105	1x105	1x104	BND	BND	BND	BND	BND	BND
	3	0.86	0.33	0.69	0.72	1x105	1x104	1x103	1x102	<10CFU	1x103	<10CFU	<10CFU	1x102
	4	0.88	0.37	0.41	0.74	1x105	1x104	1x103	1x102	<10CFU	1x103	1x101	<10CFU	1x101
	5	BND	0.31	0.44	0.46	1x105	1x104	1x104	BND	BND	BND	BND	BND	BND
	6	BND	0.28	0.43	0.51	1x105	1x104	1x104	BND	BND	BND	BND	BND	BND
	7	BND	0.27	0.42	0.55	1x105	1x104	1x104	BND	BND	BND	BND	BND	BND
	8	BND	0.22	0.34	0.52	1x105	1x104	1x104	BND	BND	BND	BND	BND	BND
	9	BND	0.18	0.33	0.47	1x105	1x104	1x103	BND	BND	BND	BND	BND	BND