Table 4.

Characteristics of Duo-Specie Biofilms of Pseudomonas aeruginosa and Staphylococcus aureus Cell surface hydrophobicity, Planktonic population and SCVs or persister cells population at pre and post biofilm stage

Combination	S #	Biofilm OD578a	Cell surface hydrophobicity			Pre-biofilm population after 24 hr of incubation		Biofilm population after 48 hr of incubation		SCVs or persister cell population of biofilms
			At 18 hr	At 24 hr	At 48 hr	P. aeruginosa	S. aureus	P. aeruginosa	S. aureus	P. aeruginosa	S. aureus
Biofilm positive isolates	1	0.97	0.23	0.49	0.88	1x104	1x105	1x103	1x101	1x102	<10CFU
	2	0.99	0.21	0.63	0.87	1x104	1x105	1x103	<10CFU	1x102	<10CFU
	3	0.93	0.21	0.47	0.81	1x104	1x105	1x103	<10CFU	1x102	<10CFU
	4	0.98	0.28	0.57	0.88	1x104	1x104	1x102	<10CFU	1x102	<10CFU
	5	0.97	0.31	0.61	0.82	1x104	1x105	1x102	<10CFU	1x102	<10CFU
Biofilm negative isolates	1	BND	0.19	0.27	0.28	1x104	1x105	BND	BND	BND	BND
	2	BND	0.18	0.27	0.31	1x104	1x105	BND	BND	BND	BND
	3	0.61	0.31	0.39	0.57	1x104	1x105	1x102	<10CFU	<10CFU	<1CFU
	4	BND	0.13	0.21	0.25	1x104	1x105	BND	BND	BND	BND
	5	BND	0.21	0.19	0.35	1x104	1x105	BND	BND	BND	BND
Biofilm positive S. aureus with biofilm negative P. aeruginosa	1	BND	0.19	0.26	0.33	1x105	1X103	BND	BND	BND	BND
	2	BND	0.21	0.28	0.31	1x104	1X104	BND	BND	BND	BND
	3	0.58	0.26	0.47	0.66	1x105	1X104	1x103	<10CFU	<10CFU	<10CFU
	4	0.66	0.19	0.49	0.73	1x105	1X103	1x103	<10CFU	<10CFU	<10CFU
	5	0.63	0.23	0.53	0.79	1x104	1X103	1x103	<10CFU	<10CFU	<10CFU
Biofilm negative S. aureus with biofilm positive P. aeruginosa	1	0.66	0.22	0.56	0.77	1x104	1X104	1X103	<10CFU	1x102	<10CFU
	2	0.81	0.22	0.49	0.81	1x104	1X104	1X103	<10CFU	1x102	<10CFU
	3	0.89	0.21	0.53	0.83	1x104	1X104	1X103	<10CFU	<10CFU	<10CFU
	4	0.82	0.23	0.52	0.84	1x104	1X104	1X103	1x102	1x102	<10CFU
	5	0.88	0.25	0.55	0.82	1x104	1X104	1X103	1x102	1x102	<10CFU